B.Pharm Pharmacological Screening Methods Notes

PHARMACOLOGICAL SCREENING METHODS UNIT-WISE NOTES – BP810ET

Unit – 1

• Laboratory Animals:
• Study of CPCSEA and OECD guidelines for maintenance, breeding and conduct of experiments on laboratory animals, Common lab animals: Description and applications of different species and strains of animals. Popular transgenic and mutant animals.
• Techniques for collection of blood and common routes of drug administration in laboratory animals, Techniques of blood collection and euthanasia.

Unit – 2

• Preclinical screening models
• a. Introduction: Dose selection, calculation and conversions, preparation of drug solution/suspensions, grouping of animals and importance of sham negative and positive control groups.
• Rationale for selection of animal species and sex for the study.
• b. Study of screening animal models for Diuretics, nootropics, anti-Parkinson’s, antiasthmatics,
• Preclinical screening models: for CNS activity- analgesic, antipyretic, anti-inflammatory, general anaesthetics, sedative and hypnotics, antipsychotic, antidepressant, antiepileptic, antiparkinsonism, alzheimer’s disease

Unit – 3

• Preclinical screening models: for ANS activity, sympathomimetics, sympatholytics, parasympathomimetics, parasympatholytics, skeletal muscle relaxants, drugs acting on eye, local anaesthetics

Unit – 4

• Preclinical screening models: for CVS activity- antihypertensives, diuretics, antiarrhythmic, antidyslepidemic, anti aggregatory, coagulants, and anticoagulants
• Preclinical screening models for other important drugs like antiulcer, antidiabetic, anticancer and antiasthmatics.

Unit – 5

• Research methodology and Bio-statistics
• Selection of research topic, review of literature, research hypothesis and study design
• Pre-clinical data analysis and interpretation using Students ‘t’ test and One-way ANOVA. Graphical representation of data