B.Pharm Advanced Instrumentation Techniques Notes

ADVANCED INSTRUMENTATION TECHNIQUES UNIT-WISE NOTES – BP811ET

Unit – 1

• Nuclear Magnetic Resonance spectroscopy
• Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin – spin coupling, relaxation, instrumentation and applications
• Mass Spectrometry- Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications

Unit – 2

• Thermal Methods of Analysis: Principles, instrumentation and applications of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA),
• Differential Scanning Calorimetry (DSC)
• X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, Xray Crystallography, rotating crystal technique, single crystal diffraction, powder diffraction, structural elucidation and applications.

Unit – 3

• Calibration and validation-as per ICH and USFDA guidelines
• Calibration of following Instruments
• Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC

Unit – 4

• Radio immune assay: Importance, various components, Principle, different methods, Limitation and Applications of Radio immuno assay
• Extraction techniques:General principle and procedure involved in the solid phase extraction and liquid-liquid extraction

Unit – 5

• Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS.