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INSTRUMENTAL METHODS OF ANALYSIS UNIT-WISE NOTES – BP701T Unit – 1
UV Visible spectroscopy: Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on
absorption spectra, Beer and Lambert’s law, Derivation and deviations.
Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
Applications – Spectrophotometric titrations, Single component and multi component analysis
Fluorimetry: Theory, Concepts of singlet, doublet and triplet electronic states, internal and external
conversions, factors affecting fluorescence, quenching, instrumentation and applications
Unit – 2
IR spectroscopy: Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations
Instrumentation – Sources of radiation, wavelength selectors, detectors – Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications
Flame Photometry – Principle, interferences, instrumentation and applications
Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications
Nepheloturbidometry – Principle, instrumentation and applications
Unit – 3
Introduction to chromatography:
Adsorption and partition column chromatography-Methodology, advantages, disadvantages and applications.
Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.
Paper chromatography-Introduction, methodology, development techniques, advantages, disadvantages and applications
Electrophoresis – Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications
Unit – 4
Gas chromatography – Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications
High performance liquid chromatography (HPLC)-Introduction, theory, instrumentation, advantages and applications.
Unit – 5
Ion exchange chromatography- Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications
Gel chromatography- Introduction, theory, instrumentation and applications
Affinity chromatography- Introduction, theory, instrumentation and applications
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